- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
ENZ-1064
Synonyms
Glutaredoxin-1, Thioltransferase-1, TTase-1, Glrx, Glrx1, Grx, Grx1.
Introduction
GLRX1 has a glutathione-disulfide oxidoreductase activity in the presence of nadph and glutathione reductase. reduces low molecular weight disulfides and proteins. Glutaredoxin is a glutathione (GSH)-dependent hydrogen donor for ribonucleotide reductase and also catalyzes glutathione-disulfide oxidoreduction reactions in the presence of NADPH and glutathione reductase. GLRX1 is multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage.
Description
GLRX Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (1-107 a.a) and having a molecular mass of 14.3kDa. GLRX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
GLRX protein solution (0.5mg/ml) containing 20mM MES (pH5.0), 20% Glycerol and 0.1M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQEFVN CKIQSGKVVV FIKPTCPYCR KTQEILSQLP FKQGLLEFVD ITATNNTSAI QDYLQQLTGA RTVPRVFIGK DCIGGCSDLI SMQQTGELMT RLKQIGALQL
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.