prospec

GARS Human, sf9

  • Name
  • Description
  • Pricings
  • Quantity
  • GARS Human, sf9
  • Glycyl-TRNA Synthetase Human Recombinant, sf9
  • Shipped with Ice Packs

Catalogue number

ENZ-717

Synonyms

Glycine--tRNA ligase, EC 6.1.1.14, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS, HMN5, CMT2D, DSMAV, SMAD1.

Introduction

GARS is an (alpha)2 dimer which is a member of the class II family of tRNA synthetases. GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases which charge tRNAs with their cognate amino acids. GARS catalyzes the attachment of glycine to tRNA(Gly). In addition, GARS is able to produce diadenosine tetraphosphate (Ap4A), which is a universal pleiotropic signaling molecule required for cell regulation pathways, by direct condensation of two ATPs. GARS has been demonstrated to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Description

GARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 78,902 Dalton.
GARS is expressed with a -10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Sf9 Insect Cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

GARS is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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