Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa), Glutamate Decarboxylase 65 KDa Isoform, 65 KDa Glutamic Acid Decarboxylase, EC 22.214.171.124, GAD-65, GAD65, Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kD), Glutamate Decarboxylase-2 (Pancreas), EC 4.1.1, GAD2.
Glutamate Decarboxylase 2 (GAD2) is one of several forms of glutamic acid decarboxylase, identified as a main autoantigen in type II diabetes. The GAD2 enzyme is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for the GAD2 enzyme has been characterized in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in type II diabetes. GAD2 gene may also have a role in the stiff man syndrome. In addition, GAD2 catalyzes the production of GABA.
Recombinant Human GAD2 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66,234 Dalton.
GAD2 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Sterile Filtered clear solution.
GAD2 protein solution is supplied in 16mM HEPES buffer pH-7.5, 250mM NaCl, 0.016mM Pyridoxal-5’-Phosphate, 0.16% Triton x-100 and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 95.0% as determined by SDS-PAGE.
1. Binds IgG type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels).
Safety Data Sheet
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.