- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-218
Synonyms
Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.
Introduction
Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The HPR -binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Description
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.2kDa.
The FGF-b is purified by proprietary chromatographic techniques.
The FGF-b is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM Tris-HCl, pH7.4 and 1M NaCl.
Solubility
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by Analysis by SDS-PAGE.
Amino acid sequence
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine balb/c 3T3 cells is <0.1ng/ml, corresponding to a specific activity of graeter than 1.0x107 Units/mg.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References
1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas
Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010
Link:FGF 2 prospec publication
2.Title:Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation
Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013.
Link:FGF 2 Human prospec publication
3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain .
Publication:
- Cell Transplantation
- ISSN: 0963-6897
- DOI: 10.3727/096368910X498944
- Volume 19, Issue 8, pages 985-998
- Copyright © 2010 Cognizant Comm. Corp
Link:Fibroblast Growth Factor-Basic prospec publication
4.Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues.
Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press.
Link:FGF-basic prospec publication
5.Title: Successful implantation of physiologically functional bioengineered mouse internal anal sphincter
Publication: American Journal of Physiology-Gastrointestinal and Liver Physiology 299.2 (2010): G430-G439.
Link: FGF-b prospec publication
Background
FGF-2 Fibroblast Growth Factor-Basic Human Recombinant: Unraveling Its Pleiotropic Roles in Development and Disease
Abstract:
FGF-2 (Fibroblast Growth Factor-Basic), also known as Basic FGF, HBGF-2, FGF-2, or FGF-b, is a multifunctional growth factor that plays diverse roles in various biological processes, including embryonic development, tissue repair, angiogenesis, and tumorigenesis.
This research paper aims to provide a comprehensive analysis of the molecular characteristics, signaling pathways, and wide-ranging physiological functions of FGF-2. Additionally, it explores the therapeutic implications of FGF-2 in different diseases and disorders.
Synonyms such as Basic FGF, HBGF-2, FGF-2, and FGF-b associated with the protein are discussed throughout the paper to highlight their relevance in scientific literature.
Introduction:
- FGF-2, also known as Basic FGF, HBGF-2, FGF-2, or FGF-b, is a growth factor with diverse roles in development and disease. This section introduces FGF-2 and its synonyms, highlighting their significance and relevance in scientific research.
Molecular Characteristics of FGF-2:
- This section explores the molecular characteristics of FGF-2, including its primary amino acid sequence, protein structure, and post-translational modifications. The importance of these factors in determining FGF-2's biological activity and receptor binding is discussed.
Signaling Pathways Activated by FGF-2:
- FGF-2 activates specific signaling pathways upon binding to its receptors, leading to diverse cellular responses. This section focuses on the activation of the MAPK/ERK, PI3K/Akt, and PLCγ pathways. The downstream effectors and transcriptional regulators involved in mediating FGF-2's cellular responses are also discussed.
Physiological Functions of FGF-2:
- FGF-2 plays critical roles in various physiological processes, including embryonic development, tissue regeneration, angiogenesis, and wound healing. This section provides an in-depth analysis of FGF-2's contributions to these processes, emphasizing its role in cell proliferation, migration, differentiation, and extracellular matrix remodeling.
Therapeutic Implications of FGF-2:
- The multifaceted properties of FGF-2 make it a promising therapeutic candidate for various diseases and disorders. This section discusses the potential applications of FGF-2 in tissue engineering, wound healing, cardiovascular diseases, and neurodegenerative disorders. The challenges and future directions in utilizing FGF-2 as a therapeutic agent are also explored.
FGF-2 in Disease Pathogenesis:
- FGF-2 is implicated in the pathogenesis of several diseases, including cancer, fibrosis, and neurologic disorders. This section examines the role of FGF-2 in promoting tumor growth, angiogenesis, tissue fibrosis, and neuroprotection. The therapeutic implications and targeting of FGF-2 in disease management are also discussed.
Conclusion:
- FGF-2, also known as Basic FGF, HBGF-2, FGF-2, or FGF-b, is a critical growth factor involved in various biological processes and disease pathogenesis. Understanding the molecular characteristics, signaling pathways, and physiological functions of FGF-2 contributes to the exploration of its therapeutic potential in different disorders.