prospec

ESAT6

  • Name
  • Description
  • Pricings
  • Quantity
  • ESAT6
  • Early Secretory Target Mycobacterium Tuberculosis Recombinant
  • Shipped with Ice Packs

Catalogue number

PRO-291

Synonyms

Early Secretory Target Mycobacterium Tuberculosis, ESAT-6.

Introduction

Mycobacterium antigen ESAT-6 has been isolated from low molecular weight fractions of the shot-term-culture filtrate (ST-CF) and it can easily be detected in tuberculosis patients.
The export of ESAT-6 which is a potent T-cell antigen, and related proteins requires a dedicated secretory apparatus that is encoded by a group of genes, several of which also code for proteins that are recognized strongly by T cells. The ESAT-6 systems can consequently be considered as immunogenicity islands and there is mounting evidence that the equivalent genes are subject to selective pressure imposed by the immune system of the host.
This antigen includes many epitopes detectable in the serum of most patients with tuberculosis (more than 90%). By the attempts to obtain the vaccine on the basis of ESAT-6 it was demonstrated that the optimization of adjuvant is very important when using the combination of dioctadecylammonium bromide and monophosphoryllipide.
Recently it was shown that ESAT-6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. The main topic in ESAT-6 using is in antibody production and in test-systems for tuberculosis elaboration.

Patent Protected Countries

The sale and/or commercial use of Recombinant ESAT-6 is prohibited in the United States of America (U.S.A), Canada, Great Britain, Denmark, France, Greece, Italy, Germany, Spain, Ireland, Portugal, New Zealand and Australia.

Description

ESAT-6 Recombinant produced in Baculovirus is a single, glycosylated, polypeptide chain containing 104 amino acids (1-95 a.a) having a total molecular mass of 11kDa.
The ESAT-6 fused to a 6 amino acid His-tag & purified by proprietary chromatographic techniques.

Source

Baculovirus.

Physical Appearance

Sterile filtered colorless solution.

Formulation

ESAT-6 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Amino acid sequence

ADPMTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFAHH HHHH.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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