Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibody solutions (100µg in total).
Purified monoclonal IgG by protein A chromatography.
*Dengue NS1 Antibody solution for gold conjugation (3.6mg/ml) in PBS and Sodium nitrate.
*Dengue NS1 Antibody solution for membrane coating (2.2mg/ml) in PBS and Sodium nitrate.
2. Conjugation at pH 8.5, conjugation time should be 6-8 hours.
3. Blocking condition: remove supernatant and re-suspend gold pellet bound with anti-dengue NS1 to OD 8.0- 8.2, add casein to the final 0.3-0.4%.
4. Treat sample pad with 50mM sodium borate, 0.5% mouse serum and 0.5% Triton X-100, pH8.0.
Lateral flow immunoassay.