The use of a purified recombinant Cytochrome P450 2D6 antigen allows the differentiation of autoimmune hepatitis from drug-induced hepatitis where transient autoantibodies to other P450 family members occur which cannot be differentiated by immunofluorescence techniques.
Cytochrome P450 2D6 Human Recombinant (also called liver/kidney microsomal antigen 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 60 kDa. The LKM1 is purified by proprietary chromatographic techniques.
LKM-1 is supplied in 17mM HEPES pH-7.5, 425mM NaCl, 1mM DTT, 1% Triton X-100 and 15% glycerol.
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative samples). 3. Immunodot analysis with positive/negative samples.
Western-Blot with monoclonal anti-LKM-1 antibody and anti-LKM-1 autoantibody- positive sample.
0.35-0.6 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Greater than 95% as determined by SDS-PAGE.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.