DPPI, Cathepsin C, CTSC
CTSC has an important role in the activation of granule serine proteases in inflammatory cells.CTSC catalyses excision of dipeptides from the N-terminus of protein and peptide substrates. Once activated by CTSC, proteases are capable of degrading extracellular matrix components, which can result in tissue damage and chronic inflammation. CTSCcomprises of 4 subunits, each composed of the N-terminal proregion fragment, heavy chain and light chains. Defects in the CTSC protein have been demonstratedto result in Papillon-Lefevre disease which is an autosomal recessive disorder.
CTSC Mouse Recombinant produced in HEK293 cells is a single, polypeptide chain containing 444 amino acids (25-462 a.a.) and having a molecular mass of 50.5kDa. CTSC is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
CTSC protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Greater than 95% as determined by SDS-PAGE.
Safety Data Sheet
> 50,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyzes 1pmole of GlyArg-AMC / minute at pH 6.0 at 37C.
Amino acid sequence
DTPANCTYPD LLGTWVFQVG PRSSRSDINC SVMEATEEKV VVHLKKLDTA YDELGNSGHF TLIYNQGFEI VLNDYKWFAF FKYEVRGHTA ISYCHETMTG WVHDVLGRNW ACFVGKKVES HIEKVNMNAA HLGGLQERYS ERLYTHNHNF VKAINTVQKS WTATAYKEYE KMSLRDLIRR SGHSQRIPRP KPAPMTDEIQ QQILNLPESW DWRNVQGVNY VSPVRNQESC GSCYSFASMG MLEARIRILT NNSQTPILSP QEVVSCSPYA QGCDGGFPYL IAGKYAQDFG VVEESCFPYT AKDSPCKPRE NCLRYYSSDY YYVGGFYGGC NEALMKLELV KHGPMAVAFE VHDDFLHYHS GIYHHTGLSD PFNPFELTNH AVLLVGYGRD PVTGIEYWII KNSWGSNWGE SGYFRIRRGT DECAIESIAV AAIPIPKL-HHHHHH