The human cytomegalovirus UL99-encoded pp28 is a myristylated phosphoprotein that is a constituent of the virion. The pp28 protein is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope. In the infected cell, pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane.
The E.Coli derived recombinant protein contains the CMV Pp28 (UL99) immunodominant regions, 130-160 amino acids.
CMV Pp28 was purified by proprietary chromatographic technique.
CMV Pp28 protein is >95% pure as determined by 10% PAGE (coomassie staining).
25mM Tris-Hcl pH 7.2, 1mM EDTA and 50% glycerol.
CMV Pp28 protein although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Immunoreactive with sera of CMV-infected individuals.
CMV Pp28 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
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