prospec

CHIKV E1

  • Name
  • Description
  • Pricings
  • Quantity
  • CHIKV E1
  • Chikungunya E1 Recombinant
  • Shipped with Ice Packs

Catalogue number

CHI-004

Introduction

Chikungunya is an infection caused by the chikungunya virus which is passed to humans by two species of mosquito of the genus Aedes: A. albopictus and A. aegypti. Animal reservoirs of the virus include monkeys, birds, cattle, and rodents. The features of the disease are a sudden onset of fever 2-4 days after exposure. The fever typically lasts 2-7 days, while the associated joint pains usually last weeks or months but sometimes years. The mortality rate is a little less than 1 in 1,000. The disease has occurred in outbreaks in Asia, Europe and the Americas since 2004. CHIKV is a single-stranded positive-sense RNA genome, 11,800 nts long which encodes 2 open reading frames. The nucleocapsid is tightly enveloped by a host-derived lipid bilayer (envelope) supporting the virus-encoded envelope proteins. 80 glycoprotein spikes are C- terminally anchored within the viral envelope. The structural polyprotein is translated from a viral sub genomic mRNA, while as the 5 structural proteins (capsid, E3, E2, 6K, E1) are translated as a single polyprotein, from which capsid (C) is cleaved off to encapsidate. The envelope polyprotein precursor E3-E2-6K-E1 is translocated to the endoplasmatic reticulum. Polyprotein is processed by host signalases, resulting in E3, E2 &  E1 forming viral hetero-trimeric spikes. The viral spikes majorly contains E2 and E1 facilitate cell receptor recognition, cell entry thru pH-dependent endocytosis and support viral budding.

Description

Recombinant Chikungunya E1 produced in E.coli having a molecular weight of 48kDa.

Purification Method

Purified by proprietary chromatographic technique.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

Sterile Filtered solution containing PBS.

Stability

CHIKV E1 although stable at 4°C for 1 week, should be stored below -18°C.

Please prevent freeze thaw cycles.

Purity

Protein is >95% pure as determined by SDS-PAGE.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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