CD72, CD72b, LYB2, B-cell differentiation antigen CD72, Lyb-2.
CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface moleculaes such as the asialoglycoprotein receptors, CD23 and the Kupffer cell recpeotr. The exposure of B cells to CD72 antibodies activates a variety of signalling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.
CD72 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 485 amino acids (117-359a.a.) and having a molecular mass of 55.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
CD72 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
CD72 protein solution (0.5mg/ml) contains 50mM Tris-HCl buffer (pH6.8), 0.2M NaCl, 2mM DTT and 50% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
ADPRYLQVSQ QLQQTNRVLE VTNSSLRQQL RLKITQLGQS AEDLQGSRRE LAQSQEALQV
EQRAHQAAEG QLQACQADRQ KTKETLQSEE QQRRALEQKL SNMENRLKPF FTCGSADTCC
PSGWIMHQKS CFYISLTSKN WQESQKQCET LSSKLATFSE IYPQSHSYYF LNSLLPNGGS
GNSYWTGLSS NKDWKLTDDT QRTRTYAQSS KCNKVHKTWS WWTLESESCR SSLPYICEMT
AFRFPDLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH
EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL
PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE
NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH.