Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, BMP6, VGR, VGR1.
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules, which can induce ectopic bone growth. Various BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were initially identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based upon its expression early in embryogenesis, BMP6 has a suggested role in early development. Moreover, the fact that the BMP6 is closely related to BMP5 and BMP7 leads to an assumption of possible bone inductive activity.
BMP-6 Human Recombinant produced in HEK cells is a glycosylated disulfide linked homodimer of two 139 amino acid polypeptide chains; each monomer is expressed as the C-terminal part of a precursor polypeptide, which contains a 20 amino acid signal peptide and a 354 amino acid propeptide.
The BMP-6 which has a total molecular weight of 26.2kDa is purified by proprietary chromatographic techniques.
The BMP-6 was lyophilized from 10mM Tris, pH 9.0 .
It is recommended to reconstitute the lyophilized BMP-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized BMP-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by analysis by SDS-PAGE and HPLC analysis.
Amino acid sequence
VSSASDYNSS ELKTACRKHE LYVSFQDLGW QDWIIAPKGY AANYCDGECS FPLNAHMNAT
NHAIVQTLVH LMNPEYVPKP CCAPTKLNAI SVLYFDDNSN VILKKYRNMV VRACGCH.
The ED50 as determined by its ability to induce alkaline phosphatase production in the ATDC-5 cell line is typically 0.03-0.06 μg/ml.
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