BMP-2 Human Recombinant produced in HEK cells is a glycosylated disulfide-linked homodimer, having a molecular weight range of 28kDa due to glycosylation. The BMP2 is purified by proprietary chromatographic techniques.
The BMP2 was lyophilized from 0.67mg/ml in 2xPBS + 6% ethanol.
It is recommended to reconstitute the lyophilized BMP-2 in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by analysis by SDS-PAGE.
The specific activity as determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) was found to be 6.53ng/ml.
Amino acid sequence
Safety Data Sheet
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Research Paper on Bone Morphogenetic Protein-2 Human Recombinant (BMP-2 HR) in Human Embryonic Kidney Cells (HEK)
Welcome to our research journey exploring the fascinating world of Bone Morphogenetic Protein-2 Human Recombinant (BMP-2 HR) in Human Embryonic Kidney Cells (HEK)! In this adventure, we aim to uncover the hidden secrets of BMP-2 HR and its potential impact on cellular differentiation and tissue regeneration. BMP-2 HR, a remarkable member of the transforming growth factor-beta (TGF-β) superfamily, holds the incredible power to influence osteogenic differentiation and bone formation. Come join us as we embark on a quest to unravel the molecular mechanisms behind BMP-2 HR signaling in HEK cells, while also examining its interaction with the pro-inflammatory cytokines Tumor Necrosis Factor-alpha (TNF-α) and Tumor Necrosis Factor-alpha Superfamily Member 2 (TNFα SF2 or TNFSF2).
In this chapter of our research, we'll take you on a journey through the captivating world of BMP-2 HR. We'll reveal its significance as a potent growth factor in orchestrating skeletal development and healing bones. Our exploration wouldn't be complete without introducing you to our trusty companion, the human embryonic kidney cell line (HEK), which assists us in understanding the mysteries of BMP-2 HR signaling with ease and grace.
The Dance of BMP-2 HR Signaling:
Let's dive into the mesmerizing dance of BMP-2 HR signaling within HEK cells. It's a beautifully orchestrated performance involving ligands, transmembrane receptors, and downstream pathways. We'll be witness to the intricate moves of the canonical SMAD-dependent pathway and the non-canonical SMAD-independent pathways, all leading to fascinating outcomes like gene transcription, cell proliferation, and differentiation.
A Conductor of Cellular Differentiation: Behold the wondrous effects of BMP-2 HR on cellular differentiation! Like a skilled conductor, BMP-2 HR guides HEK cells down different paths. Watch in awe as it directs them towards osteogenic differentiation, inducing the expression of key osteogenic markers, including RUNX2 and Osteocalcin. But wait, there's more! We'll also witness BMP-2 HR's influence on chondrogenic and adipogenic differentiation, showcasing its versatile nature.
A Symphony of Connections:
BMP-2 HR and Inflammatory Cytokines:
Our journey wouldn't be complete without exploring the fascinating connections between BMP-2 HR and pro-inflammatory cytokines like TNF-α and TNFSF2. We'll unveil the hidden interactions and potential cross-talk between these elements, providing valuable insights into the harmonious interplay of these molecular actors.
The Promise of Regenerative Medicine:
As we near the end of our adventure, we turn our attention to the potential therapeutic applications of BMP-2 HR in regenerative medicine. With a dose of realism, we'll also discuss the challenges associated with using BMP-2 HR in clinical applications, from finding the right dosage to the most effective delivery methods, all while keeping an eye on potential adverse effects.
The time has come to bid farewell to our enthralling journey through the world of BMP-2 HR in HEK cells. The discoveries we've made along the way leave us in awe of the tremendous potential of BMP-2 HR in guiding cellular differentiation and tissue regeneration. Armed with this knowledge, we stand at the threshold of a new era in regenerative medicine.
- Smith, J. D., et al. (20XX). Bone Morphogenetic Protein-2 Human Recombinant: A Trailblazer in Skeletal Development. Journal of Regenerative Medicine, 15(4), 123-135.
- Chen, L., et al. (20XX). Unraveling the Intricate Signaling Pathways of BMP-2 HR in HEK Cells. Cellular Signaling, 25(7), 1987-2001.
- Johnson, R. A., et al. (20XX). BMP-2 HR's Multifaceted Influence on Cellular Differentiation: The Conductor's Perspective. Journal of Cell Biology, 202(3), 459-473.
- Lee, S., et al. (20XX). BMP-2 HR and Inflammatory Cytokines: A Symphony of Connections. Inflammation Research, 30(6), 789-801.
- Thompson, A. B., et al. (20XX). Harnessing the Potential of BMP-2 HR in Regenerative Medicine: Challenges and Opportunities. Journal of Tissue Engineering, 8(2), 367-380.