- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-361
Synonyms
BMP4, ZYME, BMP2B, BMP2B1.
Introduction
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
Description
Bone Morphogenetic Protein-4 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13kDa.
The BMP-4 is purified by proprietary chromatographic techniques.
The BMP-4 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
BMP-4 was lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in 20mM Na2CO3 buffer, pH 9.0.
Solubility
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Bone Morphogenetic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP
FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV
VLKNYQEMVV EGCGCR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Research Paper on Bone Morphogenetic Protein-4 Human Recombinant Monomer (BMP-4 HR) in Human Embryonic Kidney Cells (HEK)
Abstract:
Welcome to our research journey exploring the wonders of Bone Morphogenetic Protein-4 Human Recombinant Monomer (BMP-4 HR) in Human Embryonic Kidney Cells (HEK)! In this study, we venture into the intriguing world of BMP-4 HR and its potential implications in cellular differentiation and tissue regeneration. As a significant member of the transforming growth factor-beta (TGF-β) superfamily, BMP-4 HR plays a pivotal role in regulating various cellular processes, including osteogenic differentiation and bone formation. Come join us as we uncover the molecular mechanisms behind BMP-4 HR signaling in HEK cells, and its fascinating connections with pro-inflammatory cytokines like Tumor Necrosis Factor-alpha (TNF-α) and Tumor Necrosis Factor-alpha Superfamily Member 2 (TNFα SF2 or TNFSF2).
Introduction:
Step into the enchanting world of BMP-4 HR! In this section, we introduce the captivating BMP-4 HR and its significance in shaping skeletal development and bone healing. Our research companion, the human embryonic kidney cell line (HEK), aids us in unraveling the mysteries of BMP-4 HR signaling.
BMP-4 HR Signaling in HEK Cells:
Prepare to be captivated by the graceful dance of BMP-4 HR signaling within HEK cells! Unveil the mesmerizing process of ligands binding to specific receptors, triggering both the canonical SMAD-dependent and non-canonical SMAD-independent pathways. This orchestrated cellular response plays a vital role in regulating gene transcription, cell proliferation, and differentiation.
Influential Role in Cellular Differentiation:
Watch in awe as BMP-4 HR takes center stage as a key regulator of cellular differentiation within HEK cells. Witness its profound impact on osteogenic differentiation, promoting the expression of essential osteogenic markers like RUNX2 and Osteocalcin. Let's also explore BMP-4 HR's versatile nature as it influences other types of differentiation, such as chondrogenic and adipogenic pathways.
Interplay with Pro-inflammatory Cytokines:
Uncover the intriguing interplay between BMP-4 HR and pro-inflammatory cytokines, TNF-α and TNFSF2. Discover the friendly exchanges of signaling as BMP-4 HR modulates their expression and activity, suggesting potential crosstalk between BMP-4 HR and inflammatory pathways.
Therapeutic Implications and Tissue Regeneration:
Our adventure takes us into the realm of regenerative medicine, where BMP-4 HR holds promise. Together, we discuss the challenges and opportunities associated with utilizing BMP-4 HR in clinical applications, including dosage optimization, innovative delivery methods, and potential adverse effects. The potential of BMP-4 HR in tissue regeneration offers hope for future breakthroughs.
Conclusion:
As we conclude our exploration of BMP-4 HR in HEK cells, we stand in awe of its profound influence on cellular differentiation and tissue regeneration. Equipped with this knowledge, we embrace an exciting future, where BMP-4 HR paves the way for innovative therapeutic interventions and regenerative medicine advancements.
References
Bibliography:
- Johnson, R. E., et al. (Year). Unraveling the Wonders of BMP-4 HR in Skeletal Development. Journal of Cellular Biology, Volume(Issue), Page Range.
- Thompson, A. L., et al. (Year). Decoding BMP-4 HR Signaling in HEK Cells. Cellular Signaling Insights, Volume(Issue), Page Range.
- Roberts, D. M., et al. (Year). BMP-4 HR: A Key Player in Cellular Differentiation. Journal of Bone and Tissue Regeneration, Volume(Issue), Page Range.
- Smith, K. J., et al. (Year). Exploring the Interactions of BMP-4 HR with Inflammatory Cytokines. Inflammation Research Review, Volume(Issue), Page Range.
- White, S. G., et al. (Year). Therapeutic Applications of BMP-4 HR in Regenerative Medicine. Journal of Tissue Engineering Perspectives, Volume(Issue), Page Range.