N-TERMINAL---Human BMP-2 (Met 1 – Arg 282) Human BMP-7 (Ser 293 – Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40kDa in SDS-PAGE under non-reducing conditions.
BMP-7 is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine, and 0.05% tween 20 pH-4.
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 1ml sterile/endotoxin free water.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Safety Data Sheet
Publication:Article first published online: 30 JUL 2009 DOI:10.1111/j.1349-7006.2009.01301.x © 2009 Japanese Cancer Association.
2.Title:In Vivo Degradation of a Novel Poly(?-Caprolactone) Invertebral Cage for Anterior Cervical Discectomy and Fusion in a Porcine Model.
Publication:Tissue Engineering and Regenerative Medicine International Society – EU Meeting -2010Galway, Ireland
3.Title:Pretreatment With Bone Morphogenetic Protein-7 (Bmp-7) Mimics Ischemia Preconditioning Following Intestinal Ischemia/Reperfusion Injury in the Intestine and Liver.
Publication:November 2008 - Volume 30 - Issue 5 - pp 532-536 doi: 10.1097/SHK.0b013e31816f20f1