Defensin-like protein 1.
Art v 1, Artemisia vulgaris (mugwort) is a common weed in the Mediterranean area. The significant allergen of mugwort pollen, is a secreted modular glycoprotein with a N-terminal defensin-like and a C-terminal hydroxyproline-rich domain. The reactivity of IgE antibodies is primarily aimed to epitopes located in the N-terminal domain.
Recombinant Art v 1 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 11,486 Dalton.
Art v 1 is expressed with a 5xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Art v 1 is supplied in 20mM HEPES buffer pH-7.9 and 6M Urea.
Avoid multiple freeze-thaw cycles.
2. Immunodot test with positive/negative sera panels.