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  • UNG
  • Uracil DNA Glycosilase
  • Shipped with Ice Packs

Catalogue number



Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.


E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).


Escherichia Coli strain that carries the UNG gene from E.coli.

Physical Appearance

Sterile Filtered colorless solution.


UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.


Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C.
Please prevent freeze-thaw cycles.

Specific Activity

The Specific Activity was found to be 5U/µl.

Unit Definition

1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.

10X UNG Reaction Buffer

200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.

Reaction Conditions

1X UNG Reaction Buffer, incubate at 37°C.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.


Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.


Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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