prospec

UNG

  • Name
  • Description
  • Pricings
  • Quantity
  • UNG
  • Uracil DNA Glycosilase
  • Shipped with Ice Packs

Catalogue number

ENZ-352

Synonyms

Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.

Description

E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).

Source

Escherichia Coli strain that carries the UNG gene from E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.

Stability

Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C.
Please prevent freeze-thaw cycles.

Specific Activity

The Specific Activity was found to be 5U/µl.

Unit Definition

1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.

10X UNG Reaction Buffer

200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.

Reaction Conditions

1X UNG Reaction Buffer, incubate at 37°C.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.

Inactivation

Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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