prospec

Taq Plus DNA

  • Name
  • Description
  • Pricings
  • Quantity
  • Taq Plus DNA
  • Taq Plus DNA Polymerase Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-309

Synonyms

Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA.

Description

Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C.

Source

Recombinant E.coli contains Thermus aquaticus polymerase gene.

Unit Definition

One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C.

Storage Buffer

20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.

Formulation

5U/µl.

10X Reaction Buffer

200 mM TrisHCI (pH 8.8)
100 mM KCI
100 mM (NH4)2SO4
20 mM Mg SO4
1% Triton X-100
1 mg/ml bovine serum albumin (BSA).

Note

All reagents, including Taq Plus, should be mixed immediately before use.

Reaction Conditions

DNA synthesis is performed in 100?l of mixture containing 20-200μm dNTPs, 0.3-1μm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA.

Optimization of DNA Synthesis

It is important to ad the reaction components in the following order1. H2O.
2. 10 x reaction buffer.
3. dNTPs.
4. DNA template and primers.
5. Taq Plus.

Stability

Stable for 5 days at 10°C, for longer period of time store at -20°C.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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