- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-333
Synonyms
Thioredoxin-2, TRX2, TRX-2, TXN-2, TXN2.
Introduction
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Description
Thioredoxin-2 Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa.
Source
Escherichia Coli.
Physical Appearance
Sterile Lyophilized Powder.
Formulation
Each mg of TRX2 protein contains 20mM phosphate buffer pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized TXN2 in sterile 18MΩ-cm H2O.
Stability
TXN2 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
TXN2 activity is assayed by measuring the change in absorbance at 650 nm at 25°C
The specific activity was found to be 3 units/mg.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.