prospec

SNRPD Human

  • Name
  • Description
  • Pricings
  • Quantity
  • SNRPD Human
  • Small Nuclear Ribonucleoprotein Polypeptide D1, D2, D3 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

PRO-997

Description

SNRPD Human Recombinant produced in SF9 insect cells, is a mixed protein complex containing the SNRPD1 (14,306 Dalton), SNRPD2 (14,773 Dalton) and SNRPD3 (14,739 Dalton) proteins.
Each protein (SNRPD1+ SNRPD2+ SNRPD3) is individually fused to a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Sf9 insect cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

SNRPD is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 80% as determined by SDS-PAGE.

Coating concentration

0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Applications

Western blot with:
a) Anti-Sm positive patient sera.
b) Monoclonal anti-hexa-His-tag antibody.

Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels).

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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