SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
The E.Coli derived 32kDa recombinant protein contains the Nucleocapsid core protein 340-390 amino acids immunodominant regions.
SARS Core protein was purified by proprietary chromatographic technique.
SARS Core protein is >95% pure as determined by 10% PAGE (coomassie staining).
50mM Tris-HCl, 60mM NaCl and 50% glycerol.
SARS Core protein is shipped at ambient temperature.
Upon arrival, store at -20°C.
Stability / Shelf Life
Five years frozen, 6 months at +4°C.
SARS Core protein is Immunoreactive with sera of SARS-infected individuals.
SARS Core antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.