sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 175 amino acids (143-316 a.a.) and having a total molecular mass of 19.5kDa. CD254 is purified by proprietary chromatographic techniques.
The protein (1mg/ml) was lyophilized with 10mM sodium phosphate, pH 7.5 & 20mM NaCl.
Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino acid sequence
MPAMMEGSWL DVAQRGKPEA QPFAHLTINA ASIPSGSHKV TLSSWYHDRG WAKISNMTLS NGKLRVNQDG FYYLYANICF RHHETSGSVP TDYLQLMVYV VKTSIKIPSS HNLMKGGSTK NWSGNSEFHF YSINVGGFFK LRAGEEISIQ VSNPSLLDPD QDATYFGAFK VQDID.
Greater than 95.0% as determined by SDS-PAGE.
Measured by its ability to induce TNF-alpha production in mouse RAW264.7 cells. The ED50 is 48-72ng/ml, corresponding to a specific activity of 2.1x104 Units/mg