prospec

PPCDC Human

  • Name
  • Description
  • Pricings
  • Quantity
  • PPCDC Human
  • Phosphopantothenoylcysteine Decarboxylase Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-011

Synonyms

FLJ14585, PPC-DC, MDS018, COAC, CoaC, Phosphopantothenoylcysteine Decarboxylase.

Introduction

PPCDC is a member of the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily which takes a part in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). The biosynthesis of CoA from pantothenate needs several steps: the phosphorylation of pantothenate, the conversion of 4’-hosphopantothenate to 4''-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC is a key player in this pathway. PPCDC converts 4''-phosphopantothenate into 4''-phosphopantetheine.

Description

PPCDC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-204a.a.) and having a molecular mass of 24.6kDa.
PPCDC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The PPCDC protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer(pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS

Purity

Greater than 95% as determined by SDS-PAGE.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. 
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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