- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-1443
Synonyms
DNA-directed RNA polymerases I, II, and III subunit RPABC3, RNA polymerases I, II, and III subunit ABC3, DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, RPB17, RPB8 homolog, hRPB8, POLR2H, polymerase (RNA) II (DNA directed) polypeptide H, RPB8, RPABC3.
Introduction
POLR2H is an essential and highly conserved subunit of RNA polymerase II which is shared by the other 2 eukaryotic DNA-directed RNA polymerases, I and III. The three eukaryotic RNA polymerases are complex multisubunit enzymes which play a vital role in the transcription of nuclear genes. POLR2H encodes an essential and highly conserved subunit of RNA polymerase II which is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.
Description
POLR2H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (1-150 a.a) and having a molecular mass of 19.5kDa.
POLR2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POLR2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
POLR2H protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.