prospec

IDH1

  • Name
  • Description
  • Pricings
  • Quantity
  • IDH1
  • Isocitrate Dehydrogenase-1 Yeast Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-289

Synonyms

Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.

Introduction

Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.

Description

Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.
The ICDH is purified by proprietary chromatographic techniques.

Source

Yeast cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

One ml of solution contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

The specific activity was found to be 115 U/mg.

Unit Definition

One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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