prospec

Involucrin Human

  • Name
  • Description
  • Pricings
  • Quantity
  • Involucrin Human
  • Involucrin Human Recombinant
  • Shipped with Ice Packs

Catalogue number

PRO-512

Synonyms

Involucrin, IVL.

Introduction

Involucrin is a soluble cytoplasmic protein precursor of the epidermal cornfied envelope that becomes cross-linked by transglutaminase during envelope assembly. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea which lacks a distinct cornfied layer. The protein is a useful marker of terminal differentiation. It is tightly linked to the onset of differentiation and first expressed in the immediate suprabasal layers of the epidermis.
Involucrin is mapped to 1q21 chromosome, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
Involucrin’s structure is composed of a conserved region of about 75 amino acid residues followed by two very variable length segments that contain glutamine-rich tandem repeats. The glutamine residues in the tandem repeats are the substrate for the tranglutaminase in the cross-linking reaction. The size of the protein varies from 285 residues (in dog) to 835 residues (in orangutan).

Description

Involucrin Human Recombinant (aa 420-578) expressed in E.coli, shows a 41 kDa band on SDS-PAGE (Including GST tag).
The Involucrin is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

Involucrin at 100µg/ml in 50mM Tris-HCl, pH7.5, 10mM L-glutathione (reduced).

Stability

Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
Please prevent freeze-thaw cycles.

Applications

• ELISA
• Inhibition Assays
• Western Blotting.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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