prospec

IGF1 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • IGF1 Human
  • Insulin-Like Growth Factor 1 Human Recombinant
  • Shipped at Room temp.

Catalogue number

CYT-216

Synonyms

Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.

Introduction

The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).

Description

Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa.
IGF-I is purified by proprietary chromatographic techniques.

Source

Escherichia Coli. 

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.0.

Solubility

It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.

Amino acid sequence

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Biological Activity

The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg.

References

1.Title:Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis.

Publication: Journal of Endocrinology (2009) 202, 287–297. 

Link: http://joe.endocrinology-journals.org/content/202/2/287.full.pdf

Application: support spermatogenesis in an androgen-independent manner (Leal et al. 2006)

2.Title:Suppression of Anoikis by SKP2 Amplification and Overexpression Promotes Metastasis of Esophageal Squamous Cell Carcinoma .
Publication:doi: 10.1158/1541-7786.MCR-08-0092 Mol Cancer Res January 2009 7; 12
Link:http://mcr.aacrjournals.org/content/7/1/12.full


3.Title:MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.
Publication:THE UNIVERSITY OF TEXAS AT ARLINGTON
May 2010
Link:http://dspace.uta.edu/bitstream/handle/10106/4918/Thevenot_uta_2502D_10646.pdf?sequence=1

 4. Title: Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines.
Publication: Journal of Cancer Molecules 5(3): 73-78, 2010.  

Background

IGF stands for insulin like growth factors, which are proteins that have a high similarity to insulin. They are part of a complicated process that uses cells to communication with the physiologic environment around them. IGF’s complex system is often called the ‘axis’. This consists of:
● Two cell-surface receptors (IGF1R and IGF2R)
● Two ligands (IGF-1 and IGF-2)
● A family of six high-affinity IGF-binding proteins.
● Proteases.

 

The Effect IGF Has On The Body
Many unique tissue types express the IGF-1 receptor, and the effects can vary. It induces the survival of neurons, may catalyse skeletal muscle hypertrophy by inducing protein synthesis, and by blocking muscle atrophy. It works as a protector for cartilage cells, and may work to be an anabolic factor for the bones. When used in high concentrations, it can activate the insulin receptor, and can even complement the effects that insulin has on the body.

 

Diseases And IGF
Diseases and IGF are closely related, and a number of them can be affected. The Insulin IGF axis is thought to have an effect on aging, with an increased life span shown in fruit flies when used in studies.
It is also important to note the crucial role that IGF plays in cancer and diabetes - IGF- 1 has been shown to stimulate growth in both prostate and breast cancer cells. The degree of risk that IGF-1 poses is up for debate, and many scientists are not in agreement. IGF has also shown to have the ability to decrease blood glucose levels, although not quite as effective as insulin.

 

How Was IGF Discovered?
Investigators began studying the effects of biological substances on cells and tissues outside the body when IGFs were discovered. The name is self explanatory in the fact that IGF performs insulin actions in some tissues, but is less potent than insulin at decreasing blood sugar. Its fundamental action is to stimulate growth, whether that be within the epidermal growth factor or the nerve growth factor.

What’s The Difference Between IGF-1 And IGF-2?
The two types of IGF are IGF-1 and IGF-2. Although the names are similar, the specific actions that they take are different - they bind and activate completely difference receptors. The major actor in them both is the effect that they have on cell growth. Most of the actions of the pituitary growth hormone are mediated by IGFs, but predominantly IGF-1. Growth hormone works to stimulate many tissues within the body, especially the liver which then secretes IGF-1. This then causes hypertrophy, or in layman's terms, an increase in cell size, as well as hyperplasia which is an increase in the number of cells.
The IGF-1 concentration will increase during childhood and hit peak during puberty, but will then decrease afterwards, as does the hormone secretion itself. It has been proven that children and adults with a deficiency of the growth hormone have low serum IGF-1 concentrations when put in comparison with others in the same age range. Patients who have conditions like acromegaly have been shown to have increased serum IGF-1 concentrations. The production of IGF-2 is less dependent on the secretion of growth hormone than IGF-1, and is much less important for stimulating linear growth.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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