Human T-lymphotropic virus (HTLV) is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma. The virus activates a subset of T-helper cellscalled Th1cells. The result is a proliferation of Th1 cells and overproduction of Th1 related cytokines (mainly IFN-gamma and TNF-alpha). Feedback mechanisms of these cytokines cause a suppression of the Th2 lymphocytes and a reduction of Th2 cytokine production (mainly IL-4, IL-5, IL-10 and IL-13). The end result is a reduction in the ability of the infected host to mount an adequate immune response to invading organisms that require a predominantly Th2 dependant response (these include parasitic infections and production of mucosal and humoral antibodies).
The E.Coli derived recombinant protein contains the gp21 immunodominant region, 351-404 amino acids, the MW is 21kDa. Fused with a GST tag at N-Terminus.
HTLV-1 gp21 was purified by proprietary chromatographic technique.
HTLV-1 gp21 is >95% pure as determined by 10% SDS-PAGE (coomassie staining) and HPLC-C4.
60mM NaCl, 50mM Tris-HCl, pH-8.0, 0.25% Sarkosil, 50% Glycerol and 10mM Glutathione.
HTLV-1 gp21 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
HTLV-1 gp21 can be used as an antigen in ELISA and Western Blots. Excellent reagent for correct detection of HTLV infections, with minimal specificity problems.
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