Hepatitis E virus (HEV), the major etiologic agent of enterically transmitted non-A, non-B hepatitis worldwide, is a spherical, non-enveloped, single stranded RNA virus that is approximately 32 to 34 nm in diameter. HEV belongs to a genus of HEV-like viruses (unassigned genus). HEV has a single-stranded polyadenylated RNA genome of approximately 8 kb. Based on its physicochemical properties it is presumed to be a calici-like virus.
The E.Coli derived HEV protein contains the HEV immunodominant regions from ORF2 452-617 a.a. having a Mw. of 44.3 kDa. HEV ORF2 protein is fused to a 26kDa GST tag and purified by standard chromatography techniques.
HEV ORF2 protein was purified by proprietary chromatographic technique.
HEV ORF2 protein is >95% pure as determined by 10% PAGE (coomassie staining).
25mM Tris-HCl, 1mM EDTA, 0.5M urea and 50% glycerol.
HEV ORF2 protein although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Immunoreactive with sera HEV-infected individuals.
HEV ORF2 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.
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