prospec

HBV AG-2 antibody

  • Name
  • Description
  • Pricings
  • Quantity
  • HBV AG-2 antibody
  • Hepatitis B Virus (AD & AY Antigens) AG-2 for Capture ELISA, Mouse antibody
  • Shipped at Room temp.

Catalogue number

ANT-039

Type

Mouse antibody Monoclonal.

Introduction

Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.

Clone

NYRHepB-2.

Immunogen

r.Hep B vaccine (BTG).

Ig Subclass

mouse IgG1.

Purification Method

Ion exchange column.

Formulation

1mg/ml in PBS (after reconstitution).

Shipping Conditions

Antibody is shipped lyophilized at ambient temperature.

Storage Procedures

In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Solubility

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Applications

Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry.

Titer

By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Note

This antibody recognizes both the AD and the AY antigens in direct ELISA and works in capture ELISA with anti-hepatitis Ag-1.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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