prospec

H3F3A Human

  • Name
  • Description
  • Pricings
  • Quantity
  • H3F3A Human
  • H3 Histone Family 3A Human Recombinant
  • Shipped with Ice Packs

Catalogue number

PRO-1452

Synonyms

RP11-396C23.1, H3.3A, H3F3, Histone H3.3, H3 Histone, Family 3A.

Introduction

H3F3A is a member of the histone H3 family. Histones are basic nuclear proteins which are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Histones play a significant role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated by a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Two molecules of each of the four core histones H2A, H2B, H3, and H4 form an octamer, around that approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.

Description

H3F3A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (1-136 a.a) and having a molecular mass of 17.7kDa.
H3F3A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

H3F3A protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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