- Name
- Description
- Pricings
- Quantity
Catalogue number
PRO-390
Synonyms
Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.
Introduction
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.
CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.
Description
CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa.
CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Sf9 insect cells.
Physical Appearance
Sterile Filtered liquid formulation.
Formulation
CENPB is supplied at a concentration of in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Coating concentration
0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Suitable for biotinylation and iodination.
Purity
Greater than 95% as determined by SDS-PAGE.
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.