Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein, Spliceosome-associated protein SPF 27, BCAS2, DAM1, SPF27, Snt309.
Pre-mRNA-splicing factor SPF27 (BCAS2) is a ubiquitously expressed nuclear protein that was initially identified as being overexpressed in various breast cancer cell lines. BCAS2 is currently as a component of the spliceosome, participating in the exclusion of introns from mRNA precursors. BCAS2 interacts in a ligand-independent manner particularly with Thyroid Hormone Receptor ? (TR?), Estrogen Receptor ? (ER?), ER?, Progesterone Receptor (PR) and Peroxisome Proliferator-activated receptor ? (PPAR?). BCAS2 acts as an ER co-activator and is capable of boosting ER-mediated transcription, suggesting that BCAS2 is involved in the development of breast cancer.
BCAS2 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 261 amino acids (1-225 a.a.) and having a molecular mass of 30.3kDa. The BCAS2 is purified by proprietary chromatographic techniques.
Sterile Filtered colorless solution.
The BCAS2 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAGT GLVAGEVVVD ALPYFDQGYE APGVREAAAA LVEEETRRYR PTKNYLSYLT APDYSAFETD IMRNEFERLA ARQPIELLSM KRYELPAPSS GQKNDITAWQ ECVNNSMAQL EHQAVRIENL ELMSQHGCNA WKVYNENLVH MIEHAQKELQ KLRKHIQDLN WQRKNMQLTA GSKLREMESN WVSLVSKNYE IERTIVQLEN EIYQIKQQHG EANKENIRQD F.
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