prospec

B220 Antibody

  • Name
  • Description
  • Pricings
  • Quantity
  • B220 Antibody
  • B220 (B cell specific isoform of CD45), Rat Anti-Mouse
  • Shipped at Room temp.

Catalogue number

ANT-139

Synonyms

LCA, PTPRC, LY5, B220, CD45R, T200, GP180, PROTEIN-TYROSINE PHOSPHATASE, RECEPTOR-TYPE C.

Type

Rat Anti Mouse Monoclonal.

Introduction

B220 (CD45R) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
B220 (CD45R) is required for t-cell activation through the antigen receptor. The first ptpase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.

Clone

mB220.

Immunogen

Purified mouse LN B cells.

Ig Subclass

Rat IgM.

Purification Method

Ion exchange column.

Formulation

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4C. After reconstitution, if not intended for use within a month, aliquot and store at -20C.

Solubility

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Applications

Staining antibody. For staining, use 10 µl/1,000,000 cells.

Available Conjugates

This antibody is only available as purified antibody.

Usage

Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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