prospec

Acrp30 Mouse, HEK

  • Name
  • Description
  • Pricings
  • Quantity
  • Acrp30 Mouse, HEK
  • Adiponectin Mouse Recombinant, HEK
  • Shipped at Room temp.

Catalogue number

CYT-435

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is an important negative regulator in hematopoiesis and immune systems. It may be involved in ending inflammatory responses through its inhibitory functions. Inhibits endothelial nf-kappa-b signaling through a camp-dependent pathway. Inhibits tnf-alpha- induced expression of endothelial adhesion molecules. Involved in the control of fat metabolism and insulin sensitivity.

Description

The Acrp30 Mouse Recombinant is fused with FLAG tag having a total Mw of 26kDa.

Source

HEK293 (Human embryonic kidney cell line).

Purity

Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed).

Biological Activity

Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1000ng/ml corresponding to a Specific Activity of 1000IU/mg. Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes.

Formulation

Filtered (0.4µm) and lyophilized from 0.5 mg/ml in PBS buffer.

Solubility

It is recommended to reconstitute the lyophilized Acrp30 Mouse in sterile 18M-cm H2O at 0.5mg/ml, which can then be further diluted to other aqueous solutions.

Stability

Store lyophilized APM-1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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