Mouse Anti Human Monoclonal Antibody.
Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.
Purified Human Vimentin.
Ion exchange column.
1mg/ml in PBS (after reconstitution).
Antibody is shipped lyophilized at ambient temperature.
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
For intra-cellular staining, use 5-10µl/1,000,000 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent.
Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry.
Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.
This antibody is also available conjugated to biotin and FITC.
Safety Data Sheet
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.