prospec

Urokinase Human

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  • Urokinase Human
  • Urokinase Human
  • Shipped at Room temp.

Catalogue number

ENZ-264

Synonyms

Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.

Introduction

Urokinase (UK) is a serine protease, which is one of biological plasminogen activators.
It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing.
It can be obtained from human urine or kidney cell culture.

Description

Urokinase is a two-chain glycoprotein containing 411 amino acids with 12 disulfide bonds. Its molecular weight is 54,000 Dalton.

Source

Human urine.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The Urokinase was lyophilized from a concentrated (1mg/ml) solution containing phosphate buffer.

Solubility

It is recommended to reconstitute the lyophilized Urokinase in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Urokinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Urokinase should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 94.0% as determined by SDS-PAGE.

Specific Activity

174,827IU/mg.

Contaminants

Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.

References

1. Title:Transient ?-catenin stabilization modifies lineage output from human thymic CD34+CD1a progenitors.
 Publication:Published online before print December 1, 2009, doi: 10.1189/jlb.0509344 March 2010 Journal of Leukocyte Biology vol. 87 no. 3 405-414 .
Link:http://www.jleukbio.org/content/87/3/405.full

2. Title: The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin
Publications:  Biochem. J 410 (2008): 157-165.
Link:  http://www.biochemj.org/bj/410/bj4100157.htm

3. Title:Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.
Publication:Biochem. J 428 (2010): 473-482.
Link:http://www.biochemj.org/bj/428/0473/4280473.pdf

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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