prospec

UBE2A Human

  • Name
  • Description
  • Pricings
  • Quantity
  • UBE2A Human
  • Ubiquitin Conjugating Enzyme E2A Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-514

Synonyms

HHR6A, HR6A, RAD6A, UBC2, UBE2A, EC=6.3.2.19, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-protein ligase A, Ubiquitin carrier protein A, RAD6 homolog A.

Introduction

The alteration of proteins with ubiquitin is a vital cellular mechanism for targeting atypical or short-lived proteins for degradation. Ubiquitination involves noT less than three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A is part of the E2 ubiquitin-conjugating enzyme family. UBE2A is necessary for post-replicative DNA damage repair. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is necessary for postreplication repair of UV-damaged DNA.

Description

UBE2A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids (1-152 a.a.) and having a molecular mass of 19.4 kDa. UBE2A protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

UBE2A Human solution containing 20mM Tris HCl pH-8, 1mM DTT, 1mM EDTA and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Back to Top