prospec

T.pallidum p47 (Partial)

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  • T.pallidum p47 (Partial)
  • Treponema pallidum p47 (Partial) Recombinant
  • Shipped with Ice Packs

Catalogue number

TRP-249

Introduction

Treponema pallidum is a gram-negative spirochaete bacterium and is considered to be metabolically crippled. There are at least four known subspecies: T. pallidum pallidum, T. pallidum pertenue, T. pallidum carateum and T. pallidum endemicum. The helical structure of T. pallidum pallidum allows it to move in a corkscrew motion through viscous mediums such as mucus. Treponema pallidum subsp. pallidum has one of the smallest bacterial genomes at 1.14 million base pairs (Mb) and has limited metabolic capabilities, reflecting its adaptation through genome reduction to the rich environment of mammalian tissue.

Description

The E.Coli derived recombinant protein is fused at the C- terminus with a 6xHis Tag and contains the T.Pallidum p47 immunodominant regions.

Source

Escherichia Coli.

Purification Method

Treponema Pallidum protein was purified by proprietary chromatographic technique.

Purity

Treponema Pallidum protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation

70mM Tris-HCl pH8.0, 50mM NaCl, 50% Glycerol and 1.5M urea.

Stability

Treponema Pallidum although stable at 4°C for 1 week, should be stored below -18°C. 
Please prevent freeze thaw cycles.

Specificity

Immunoreactive with sera of T.Pallidum infected individuals.

Applications

Treponema Pallidum protein is suitable for ELISA and Western blots, excellent antigen for detection of T.Pallidum with minimal specificity problems.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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