DNA topoisomerase 1, EC 22.214.171.124, DNA topoisomerase I, TOP1, Scl-70.
TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
Bovine DNA Topoisomerase-I shows multiple bands between 76-109 KDa and is purified from bovine tissues by proprietary chromatographic techniques.
TOP1 is supplied in 20mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative samples) and immunodot test with positive/negative samples.
Western blot with anti TOP1 autoantibody positive sample.
0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Greater than 95% as determined by SDS-PAGE.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Safety Data Sheet
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.