Thrombin

Thrombin

Natural Proteins

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Description

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  • Name :

    Thrombin

    Description:

    Human Thrombin

    Product # :

    PRO-339

    Price :

    Quantity :

    Shipping Method :

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    Shipped at Room temp

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  • description
  • source
  • formulation
  • biological activity
  • More Info

Description

Thrombin was purified from human plasma.

Source

Human Plasma.

Formulation

Lyophilized protein containing mannitol, sodium chloride, calcium chloride.

Biological Activity

The specific activity was found to be 116 US Units/mg protein.

More Info

  • Introduction

    Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

  • Physical Appearance

    lyophilized powder.

  • Stability

    Lyophilized Human Thrombin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution human thrombin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized human thrombin in sterile a 0.9% NaCl solution at 500U/ml.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Thrombin Human

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