TGF-β 3 Mouse Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 113 amino acid chains and having a total molecular mass of 25.7kDa.
The TGF-β 3 is purified by standard chromatographic techniques.
The Mouse TGFB3 protein solution contains 20% Ethanol and 10mM Acetic acid.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.
1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.