TaqDNA

TaqDNA

DNA Polymerase

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  • Name :

    TaqDNA

    Description:

    Taq DNA Polymerase Recombinant

    DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1.

    Product # :

    ENZ-308

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    Quantity :

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  • description
  • source
  • formulation
  • purity
  • More Info

Description

Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

Source

Recombinant e.coli contains Thermus aquaticus polymerase gene.

Formulation

Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.

Purity

Greater than 95.0% as determined by SDS-PAGE.

More Info

  • Synonyms

    DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1.

  • Stability

    Stable for 5 days at 10°C, for longer period of time store at -20°C.

  • Specify Your Own Reaction Conditions

    Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2.

  • Storage Buffer

    Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.

  • Unit Definition

    One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200µm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10µg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Taq Dna Polymerase

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