prospec

SERPINA4 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • SERPINA4 Human
  • Kallistatin Human Recombinant
  • Shipped at Room temp.

Catalogue number

PRO-2036

Synonyms

Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 4, PI4, KST, Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4, Peptidase Inhibitor 4, Kallikrein Inhibitor, Serpin A4, PI-4, Protease Inhibitor 4 (Kallistatin), Kallistatin, KLST, KAL, SERPINA4.

Introduction

Kallistatin (SERPINA4) inhibits human amidolytic and kininogenase activities of tissue kallikrein. This inhibition is attained by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and production of a small C-terminal fragment of the inhibitor as a result of cleavage at the reactive site by tissue kallikrein.

Description

SERPINA4 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Gln21-Pro427) containing a total of 417 amino acids, having a calculated molecular mass of 47.7kDa and fused to a 10 aa His tag at C-Terminus.

Source

HEK 293.

Physical Appearance

Filtered White lyophilized (freeze-dried) powder.

Formulation

SERPINA4 was filtered (0.4µm) and lyophilized from 0.5mg/ml solution in phosphate buffered saline pH 7.4 and 5% (w/v) Trehalose.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. SERPINA4 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

QLHVEHDGES CSNSSHQQIL ETGEGSPSLK IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN RPFLVVIFST STQSVLFLGK VVDPTKPHHH HHHHHHH.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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