prospec
SAE1/SAE2 Human

SAE1/SAE2 Human

  • Name
  • Description
  • Cat#
  • Pricings
  • Quantity
  • SAE1/SAE2 Human

  • SAE1/SAE2 Human Recombinant
  • ENZ-1161
  • Shipped with Ice Packs

Catalogue number

ENZ-1161

Introduction

SAE1 and SAE2 form heterodimers which take part in the posttranslational modification of proteins (sumoylation). This process regulates protein structure as well as intracellular localization of the target. SAE1/SAE2 may indicate on dermatomyositis (DM) as autoantibodies against those 2 proteins have been found in patients.

Description

SAE1/SAE2 Human Recombinant produced in SF9 is glycosylated, polypeptide chain containing 2 subunits (SAE1 subunit molecular mass is 39kDa & SAE2 subunit molecular mass is 73kDa). The subunits associate to form a complex.
The SAE1/SAE2 is expressed with a -10xHis tag and purified by proprietary chromatographic techniques.

 

Source

Sf9 insect cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

SAE1/SAE2 is supplied in 20mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative samples) and immunodot test with positive/negative samples.

Applications

Western blot with anti SAE1/SAE2 autoantibody positive sample & Polyclonal anti-SAE1 and anti-SAE2 antibodies.

Coating concentration

0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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