prospec

S100A1 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • S100A1 Human
  • S100 Calcium Binding Protein A1 Human Recombinant
  • Shipped at Room temp.

Catalogue number

PRO-364

Synonyms

Protein S100-A1, S100 calcium-binding protein A1, S-100 protein alpha subunit, S-100 protein alpha chain, S100A1, S100A, S100, S100-alpha, S100-A1.

Introduction

S100A1 is a member of the S100 family of calcium binding proteins with EF-hand type Ca+2 binding motive. S100A1 (Calcium Binding Protein A1) is involved in the activation of sarcoplasmatic calcium release and the regulation of intermediate filament polymerization. S100A1 may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of S100A1 has been implicated in cardiomyopathies.
S100 proteins are localized either in the cytoplasm or the nucleus of a wide range of cells. There are at least 13 members in the S100 gene family, which are located as a cluster on chromosome 1q21.

Description

S100A1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids which include a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 11.66 kDa.
S100A1 Human Recombinant is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

White lyophilized (freeze-dried) powder.

Formulation

The S100A1 protein was lyophilized from 0.4µm filtered solution at a concentration of 0.5mg/ml containing 20mM Tris pH-7.5 and 50mM NaCl.

Solubility

Add deionized water to a working concentration approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.

Stability

Lyophilized S100A1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution S100A1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Amino acid sequence

MKHHHHHHAS GSELETAMET LINVFHAHSG KEGDKYKLSK KELKELLQTE LSGFLDAQKD VDAVDKVMKE LDENGDGEVD FQEYVVLVAA LTVACNNFFW ENS.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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