- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2822
Synonyms
SLC42A5, CD240D, DIIIc, RhPI, RhII, Rh4.
Description
The RhD Human is created as a recombinant protein with a 4kda N-terminal fusion of His Tag.
The RhD His-Tagged Fusion Protein, produced in E. coli, is a 13kDa protein containing 58 amino acid residues of the RhD Human, 108-165 amino acids.
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
Each mg was lyophilized with 1xPBS, 0.4% SDS and 4mM DTT.
Solubility
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile!
Please filter the product by an appropriate sterile filter before using it on cell culture.
Stability
Store lyophilized RhD at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity
Greater than 90% as determined by SDS-PAGE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
The Rh blood group system is one of the major blood group systems in humans and the D antigen is the main component of this system.
D Antigen
The presence of the D antigen on the surface of red blood cells indicates a positive Rh factor. Individuals with this antigen are classified as Rh-positive (Rh+), while those without it are Rh-negative (Rh-).
Genetics
The Rh factor is inherited in a Mendelian manner, with the D antigen being monitored by the RHD gene located on chromosome 1. The presence of the D allele (RHD) results in Rh positivity.
Immunization
Rh-negative individuals can develop antibodies against the D antigen if exposed to Rh-positive blood. In order to prevent such interaction, Rh-negative mothers are often given Rh immunoglobulin (Rho(D) immune globulin) during and after pregnancy.
Clinical Significance
The Rh D antigen is vital in blood transfusions and pregnancy. Rh incompatibility can occur when an Rh- mother carries an Rh+ fetus, which may lead to hemolytic disease of the newborn (HDN).
Blood Transfusions
Rh compatibility is very important in transfusions. Rh- individuals should receive Rh- blood to avoid an immune reaction.