prospec

sRANKL Mouse

  • Name
  • Description
  • Pricings
  • Quantity
  • sRANKL Mouse
  • RANK Ligand Soluble Mouse Recombinant
  • Shipped at Room temp.

Catalogue number

CYT-320

Synonyms

Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf.

Introduction

RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Description

sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 175 amino acids (143-316 a.a.) and having a total molecular mass of 19.5kDa. CD254 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein (1mg/ml) was lyophilized with 10mM sodium phosphate, pH 7.5 & 20mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino acid sequence

MPAMMEGSWL DVAQRGKPEA QPFAHLTINA ASIPSGSHKV TLSSWYHDRG WAKISNMTLS NGKLRVNQDG FYYLYANICF RHHETSGSVP TDYLQLMVYV VKTSIKIPSS HNLMKGGSTK NWSGNSEFHF YSINVGGFFK LRAGEEISIQ VSNPSLLDPD QDATYFGAFK VQDID.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Biological Activity

Measured by its ability to induce TNF-alpha production in mouse RAW264.7 cells. The ED50 is 48-72ng/ml, corresponding to a specific activity of 2.1x104 Units/mg

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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