sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 160 amino acids (158-316 a.a.) and having a total molecular mass of 17.9kDa. CD254 is purified by proprietary chromatographic techniques.
The sRANKL Mouse was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.0, 200mM NaCl and 0.1mM EDTA.
Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino acid sequence
MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The ED50 as determined by its ability to induce osteoclast differentiation of murine RAW 264.7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.