Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein, PSMB9, LMP2, PSMB6i, RING12, beta1i, MGC70470.
Proteasome subunit beta type-9 (PSMB9) belongs to the proteasome B-type family which is a 20S core beta subunit. PSMB9 is positioned in the class II region of the MHC (major histocompatibility complex). Expression of the PSMB9 protein is induced by INFG and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome.
PSMB9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (21-219 a.a.) and having a molecular mass of 23.5kDa.
PSMB9 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Sterile filtered colorless solution.
PSMB9 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 50mM NaCl.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MTTIMAVEFD GGVVMGSDSR VSAGEAVVNR VFDKLSPLHE RIYCALSGS AADAQAVADMA AYQLELHGIE LEEPPLVLAA ANVVRNISYK YREDLSAHLM VAGWDQREGG QVYGTLGGML TRQPFAIGGS GSTFIYGYVD AAYKPGMSPE ECRRFTTDAI ALAMSRDGSS GGVIYLVTIT AAGVDHRVIL GNELPKFYDE.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 90.0% as determined by SDS-PAGE.
Safety Data Sheet
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.