The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC DNAman computer analysis program of protein sequences.
Safety Data Sheet
Publication:Published online before print December 18, 2008, doi: 10.1210/en.2008-1318 Endocrinology April 1, 2009 vol. 150 no. 4 1646-1653 .
2.Title:ISLET ENDOTHELIAL CELLS AND PANCREATIC ?-CELL PROLIFERATION: STUDIES IN VITRO AND DURING PREGNANCY IN ADULT RATS.
Publication:Endocrinology. First published January 26, 2006 as doi:10.1210/en.2005-0997.
3.Title:Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.
Publication:Endocrinology. First published ahead of print December 18, 2008 as doi:10.1210/en.2008-1318.